TPOVU
/<Pu+
*b2mU
DrawMap ver 2.2 TIFF file (
\drawmap
Graphics error:  Drawing the picture into memory."To interrupt drawing, press a key.;When the picture appears, use the arrow keys to move around)and the <ESC> key to return to main menu.5Due to memory limitations, only part of the picture (
%) will be drawn.	
P F16 F1
SP 1;
%!PS-Adobe-2.0 EPSF-2.0
%%BoundingBox: 37 105 555 798
1 setlinecap
1 setlinejoin
*t75R
*t150R
*t300RU
SP 0;
stroke
showpage
T4 1000 1000
W 0 2000 0 2000
IP 3860, 3860, 7460, 7460;
SC 0, 1000, 0, 1000;
297 538 translate
0.255 0.255 scaleU
W 2030 2790 0 2000
stroke
 lineto
 movetoU
f6Y^_
stroke
 setlinewidthU
JPA, PU,-1000,-1000, PD,-1000,1000, 1000,1000, 1000,-1000, -1000,-1000, PU;U
GPA, PU,1035,-1000, PD,1035,1000, 1680,1000, 1680,-1000, 1035,-1000, PU;LPA, PU,-1000,-1035, PD,1000,-1035, 1000,-1680, -1000,-1680, -1000,-1035, PU;U
4RSP1
)The map is quite dense. Consider reducing the site character size down to 
 % ..The map is very dense. The site character size
will be reduced down to U
 Drawing the picture into memory.(Hit any key when your plotter is ready !/To interrupt drawing, press a key.             U
Output into file.
Please enter filename
 (<return> for 
   Illegal filename.#   Existing file. OK to overwrite? 
\DRAWMAP
Nothing to draw !
\dmbatch.log
\*.mapN   <ESC> - cancel                          <any other key> - start drawing    
No files
One file
 files	and more!O   RUNNING !                            <any key> - interrupts drawing soon    
          time: 
total time spent was 
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F Please provide a proper filename (with an optional pathname) for the ; file where your DNA sequence is. The current directory is 	\DRAWMAP.B The sequence file contains something that is composed of other   B characters than 'ACGT' (it might be a title for the sequence).   B Press 'Y' or <return> if this string should be ignored.          B If it will not be ignored, the 'ACGT'-characters within it       B are considered as a part of your sequence.                       A It is possible that, inspite of the overflow, all of your bases A were recorded. You can proceed with the bases actually read.    4 The hesitation is due to the 'other characters'.   4 Abandoning leads to reading another sequence file. 4 You can choose from entering the enzymes and their 4 recognition sequences either from a file (F) or    4 from the keyboard (K). In the latter case you can  4 store the set into a file.                         > Please provide a proper filename (with an optional pathname) > for the file where your restriction enzymes are with their   2 recognition  sequences. The current directory is 
\DRAWMAP. > Note: If you give a filename with no period (.), the name    >       will be extended with the extension .ENZ               7 Free format, also blank accepted. (max 20 characters) 7 Please provide with the recognition sequence for the  7 enzyme named above. In addition to A, C, G and T, you 7 can use the following caharacters;                    
 P =  A or G     
 Q =  C or T     
 R =  A or T     
 S =  C or G     
 V =  A or C     
 W =  G or T     
 N =  A,C,G or T < You can save your enzyme set for later use by giving it a  < proper filename (with a path) by which you refer it later. 
 The current directory is  \DRAWMAP.                       < Note: If you give a filename with no period (.), the name  <       will be extended with the extension .ENZ             4 Answer 'Y' if you decide to destroy the old file   4 with this name.                                    ? Answer  'N' if  you  want  to  scan  only  the  upper strand. ? Makes a difference with nonsymmetrical recognition sequences. U
*.enz
% You are interrupting this section ! % Please confirm by pressing ESC      %                                     U
9    The display shows now the number of recognition site 9    matches in your sequence for each of the restriction 9    enzymes tested.  The 'active'  enzymes will be shown 9    on your circular plasmid map or in the comment field 9    (the latter takes place if there are  no matches for 9    the recognition sequence).  The 'inactive' sites are 9    ignored.  The enzyme names are  color coded  in  the 9    following way:                                       9    no. of matches:   0      more                        
                     
Pst1    
active              
inactive            9    There is  an upper limit  for  the number of  active #    sites to be drawn on your map (
 total).           9    You can move  the cursor to  each of  the enzymes by 9    using  the arrow keys.  The state of  the enzyme  at 9    the cursor  can be  changed by hitting the following 9    keys:                                                
       	space bar)      change the state                   
             
 + )      change to active                   
 - )      change to inactive                 9    You can also make categorial changes by hitting keys 9    introduced on the following page...                  9    Hitting the following keys make changes to the whole 9    set of enzymes:                                      
 9 +    inactivate  all  enzymes  where  more  9                  recognition  site  matches  have been  9                  found than the key indicates           
           
 * +    inactivate  all  enzymes  with  no     9                  recognition   site   matches           
 / +    activate all enzymes                   U
.  <ESC> - back to selecting the enzymes       
 - page up and down 
k<3u(
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l<Su	
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R<Wu	
E<1u	
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total 
 sites.               
(max 
 allowed for ESC)U
\DRAWMAPP     <F1> - info                         <ESC> - back to the main menu          BIn this section you can start constructing your plasmid map from a@DNA sequence and search restriction enzyme cleavage sites in it.
There is enough memory for 
 bases.1Please enter the filename for your sequence file.
Press <return> for <No such file found. Please give a name for an existing file.
dmap.seq
.Disk space limiting. Sequence will be partial.
(Should the following string be ignored ?
It will be.
It won't be.
Reading . . . 
 bases&The sequence does not fit into memory.-Removing of any memory resident programs will&allow larger sequences to be analyzed.  bases have been read from file #Do you want to continue with these 
 bases ?.Sequence statistics (only bases are recorded):
  bases
  blanks	  numbers
  other characters
, such as: 
  occurences of: 
Abandon the sequence ?1Do you want to enter the restriction enzymes from/the keyboard or from a file ?   (Answer K or F)
"Please enter the enzyme file name.
.enz5Enter the names for the enzymes and their recognition0sequences. Stop with empty recognition sequence.
\DRAWMAP\TEMP$$$$.ENZ
Enzyme  name:   
recog sequence: 
NO MORE ENZYMES, PLEASE !:If you want to save this set on disk for later use, please2give it a filename. Otherwise press only <return>.%A file with this name already exists.
Do you want to overwrite it ?.
This is not a proper filename.
(Sorry, no drive names here.)
Please give a better one.:Shall we match the recognition sequences on both strands ?
Press <return> for 'yes'
\DRAWMAP\TEMP$$$$.SIT
******
Searching: 
SITE SCANNING IS INTERRUPTED !
 ENZYMES SCANNED.
Please press slash (/)...?Returning to the main menu...                                  
                        
noname.map-The following enzymes do not cut the plasmid:U
<At,<Ct(<Gt$<Tt <Nt
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 plasmid name: P <F1> -info     <F2> -enzymes     <F3> -gel     <ESC> -back to the main menu    U
<  You are now in a section  where you  can easily  calculate<  what kind of  fragments  a restriction enzyme  digest will<  produce from  your  workfile plasmid.   (The name  of  the<  plasmid is shown highlighted at the bottom of the screen.)<     You can use up to ten enzymes in  a combined digestion.<  These enzymes are matched against  the names of  the SITES<  in the plasmid map.  A match (which leads to  a cut)  will
  be scored if:&  1) The type of the site is 'enzyme'.7  2) The name of the site contains any of the digesting(     enzyme names given in this section.<  The  principle 2) means that  the  digesting enzyme 'Sal1'<  will match agaist a site with the name  'Acc1 Sal1 Hinc2'.<  It also means that  the digesting  enzyme 'R1'  will match<  against the site 'EcoR1'.  To distinguish between 'R1' and5  'R1*' write 'R1 ' (i.e. include a blank character).<      The  principle also  means  that  the  spelling of the<  digesting enzymes given  here must be  exactly  similar to<  the site names in the plasmid map. A list of all different<  site names on your workfile map  is given when you hit the<  function key <F2>.  You can review this list at  any time.<  The list  shows  highlighted  those enzymes  which matched/  against your latest set of digesting enzymes.U
- <ESC> - back to the restriction data mode   
 - page up and down  
     U
- <ESC> - back to the restriction data mode   
 - page up and down  C             Please  stick  to  this  spelling  !                  
6 You can choose between entering the data either from 6 a file (F) or from  the keyboard (K).  In the latter 6 case you can store the set into a file.              > Please provide a proper filename (with an optional pathname) ( for the data. The current directory is 
\DRAWMAP.           > Note: If you give a filename with no period (.), the name    >       will be extended with the extension .STD               ' Free text format (max 20 characters). * Enter positive numerical values, please. = You can save your standard set for later use by giving it a = proper filename (with a path) by which you refer it later.  
 The current directory is !\DRAWMAP.                        = Note: If you give a filename with no period (.), the name   =       will be extended with the extension .STD              4 Answer 'Y' if you decide to destroy the old file   4 with this name.                                    U
*.std
+Something wrong in your standard file (row U
O     <F1> - info                                  <ESC> - back to the gel      ;Now you can change the size standard for the simulated gel.;Do you want to take the new standard from a file or load it)now from the keyboard ?   (Answer K or F)
Please enter the file name.
( <return> for 
.std<No such file found. Please give a name for an existing file.'Give below a heading for the digestion.?Now enter, one by one, the sizes in base pairs of the fragments;in your standard set. End by a <return> from an empty line.:If you want to save this set on disk for later use, please2give it a filename. Otherwise press only <return>.%A file with this name already exists.
Do you want to overwrite it ?
This is not a proper filename.
Please give a better one.U
 ESC  - back to digest more
  - stretch the gel
  F2  - new size standardU
\drawmap
Graphics error: U
W<Pu"
     U
LPT1AIn this section you can check what kind of fragments a particularA(combined) enzymatic digestion  will produce  from  your workfile
plasmid.CUse <F2> to see a list of the spelling for the enzymes on your map.
1Do you want to have the fragment lists printed ? 
fragments only
$Be sure that your printer is ready !8Please enter the enzymes of your digestion separately on6different lines. End with <return> from an empty line.6Press <return> only after typing a name for an enzyme.
Try again.
No sites for these enzymes."fragments              ends       	fragments
 	         
  to  U
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S8SESJSM  <F1> - info        <F2> - clone               <ESC> - back to the main menu
\DRAWMAP
circular
 linear 
enzyme
marker
suppressed
  active  U
X<?uJ
	 	5	H	V	~	
Save unsuccesful !
 K free space on disk./Please switch onto a disk with more free space.
Press a key ...
error U
*.map
>                                                              
        0 - 1uit the program                                  
        1 - Draw a *icture of the workfile plasmid            
        2 - Show ,estriction data from the workfile plasmid   
        3 - Get a new 'orkfile                                $        4 - Prepare a workfile from 
NA sequence              
        5 - 1dit the workfile                                 
        6 - 1ave the workfile                                 
        7 - 1hange the default drive and file directory       
 Batch >        D R A W M A P      M E N U                            >        --------------------------                            J                                                                          
            U
O  <F1> - info                                   <ESC> - back to the main menu  
 Batch -                   D R A W I N G      M E N U-                   --------------------------;       Please choose the plotter device from the list below
       
       B - 
batchU
Workfile not saved. Ok to exit?"Workfile not saved. Ok to proceed?
Draw= Choose the plotter from the above list by pressing a number.= If you want to draw all maps in the current directory, press
 first B for batch.
Inform+Your workfile does not represent a plasmid.
 <F1> - info: .map files 
Note: workfile not saved"Enter filename (return for cancel)
This is an illegal filename.
This is a new file.
This is an existing file.4This is an existing file in the specified directory.7The specified directory does not contain this map file.
Please enter again.
\DRAWMAP
Search
Nothing to save !,Illegal filename - Please give a better one.2Be kind enough to provide with an unused filename.
Existing file. OK to overwrite?
backup.$$$
Saving 
Drawmap version 	 map file
lastmap.bak Enter new (drive and) directory.1 Choose from the above list by pressing a number 1 or a key corresponding to  a highlighted letter U
<duh1
<eum1
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+!,&,J,O,s,x,
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.+.4.9.Z._.m.
/$/./3/8/B/G/n/s/-File not suitable to be interpreted as a map.4The file you indicated on disk is not suitable to be1copied as your workfile and it will be abandoned.
S = /
press a key ...
\DRAWMAP
UNEXPECTED END OF FILE;U
	Warning !
This program is version 
 but your map file
was written by the version 
TRUEU
7	]	b	l	
Screen - circle only
Screen - full drawingU
\DRAWMAP
\OUTFILE
\DRAWMAP\ALL.ENZ
\DRAWMAP\L-PST.STD
not used
DRAWMAP.INI	Warning !
This program is version 
 but your start file
was written for the version 
press a key ...
Attention !-Default settings for colors etc will be used.%For customization, run DRAWMAP SETUP.U
A                                                                 AMenu choices                                                     AHighlighted letter (hot key)                                     AMenu choice in use                                               ASubmenu choice in use                                            ACommunication with the user                                      ABox                                                              ASpecial key info                                                 AInfo line                                                        AWAIT prompt                                                      ASELECT prompt                                                    AFile list headers                                                AFile list                                                        ANumber of files found                                            U
A                                                                 AHeadings and menu choice numbers                                 AMenu choices                                                     AShort info text (also elsewhere in the program)                  ACommunication with the user                                      AInfo line                                                        U
A                                                                 ACommunication to the user                                        AUser response                                                    AReminder for printer connection                                  AResult of the digestion                                          JInfo line                                                                 AInfo line 2                                                      APlasmid name                                                     U
A                                                                 AFrame of the box                                                 AInfo text                                                        U
A                                                                 AFrame (top)                                                      AFrame                                                            AFrame (bottom)                                                   AEnzymes                                                          AEnzymes (highlighted)                                            ANumber of sites in the plasmid                                   U
A                                                                 ACommunication with the user                                      AUser response                                                    AInfo line                                                        AFile list headers                                                AFile list                                                        ANumber of files found                                            U
A                                                                 ACommunication with the user                                      AUser response                                                    AHighlighted text                                                 ARecheck for pressing  <ESC>                                      AInfo line                                                        AFile list headers                                                AFile list                                                        ANumber of files found                                            U
AInfo line (choose these colors in section 4. !)                  A                                                                 ANumber of sites in sequence (highlighted for cursor position)    ANumber of sites in sequence                                      bActive (highlighted for cursor position)                                                          bActive, but no sites (highlighted for cursor position)                                            bInactive (highlighted for cursor position)                                                        bInactive, no sites (highlighted for cursor position)                                              AActive                                                           AActive, but no sites                                             AInactive                                                         AInactive, no sites                                               AStatus line                                                      U
A                                                                 AColor code (can be changed in section 4.1)                       kColor code (can be changed in section 4.1)                                                                 EText                                                                 AKey symbols                                                      AFrame                                                            U
A                                                                 AHeadings                                                         ABoxes                                                            ABad data                                                         AComments or brief info                                           AData                                                             AMost recent input of user                                        AInfo line                                                        AWarning                                                          U
A                                                                 ABox                                                              AText                                                             AKeys                                                             U
A                                                                 AInfo line                                                        AHeadings and menu choices                                        AMenu choice in use                                               ACommunication with the user                                      AError indicator                                                  AUser response                                                    U
@                                                                @Chosen directory                                                @ .map file list                                                 @Active file                                                     @Info line                                                       @No. of files indicator and general background                   U
Cursor at: U
L          C O L O R      C U S T O M I Z A T I O N                          
Cursor at: >  arrows - to move around                 <F1> - change colors>   <F2>  - constant/chosen colors        <ESC> - done         U
                                 
2  arrows - move to the choice       <RET> - choose
  <ESC>  - cancel
3<<u"
 ESC  - back to digest more
  - stretch the gel
  F2  - new size standardU
Lambda Pst1
pBR322 EcoR1
       805
      1159
      1700
      2139
      2561
      2840
      4505
      5080
     14055
4363U
\drawmap
Graphics error: L
L          C O L O R      C U S T O M I Z A T I O N                          
;  arrows - change colors              <ESC> - done         
#DEFAULT DIRECTORIES AND FILE NAMES:$Default directory for the map files:>
                                                            
,Default file name for the DNA sequence file:'Default file name for the enzymes file:,Default file name for the DNA standard file:1          <RET> - move               <ESC> - done
ASSIGNED DRIVERS:
       
 - ,arrows - move         <RET> - assign or edit"<F1>   - unassign     <ESC> - doneU
 ---> 
      U
Basic                         
Unknown                       
Portrait                      
Landscape                     
Low resolution                
High resolution               
Extreme resolution            
 75 dpi (dots per inch)       
150 dpi (dots per inch)       
300 dpi (dots per inch)       
COM1:
COM2:U
!Pick below the device for driver 
       3arrows - move      <RET> - pick      <ESC> - cancel
 ---> 
      
PLOTTER DRIVER PARAMETERS:O             
ODescription: 
                                                                
O             
H             
  serial ports:   COM1,2JDevice:      
                                 
  parallel ports: LPT1,2,3E             
  nul device:     NULA                                                  file: any other
             
?Baud rate:   
            
  baud, parity, data bits, stop bits
             
-             
7Model:       
                              
  press 1--             
             
 Pause:       
  press Y or N
             
"                      <ESC> - done"<RET> - move          <ESC> - done
                           ?                                                               
ERROR !
Screen - circle only
Screen - full drawing
Screen - high resolution
1200,E,7,1
BBC SE284 plotter
HPGL compatible plotter
Epson compatible matrix printer$LaserJet II compatible laser printer
HP DeskJet or DeskJet Plus
PostScript printer
TIFF file (no compression) TIFF file (PackBits compression)*You are running a demo version of DrawMap.*The hard copy devices cannot be installed.
Driver * will be unassigned. Please confirm (Y/N):
P      
     1.  Menu                           
  arrows - move around          P      
     2.  Driver selection               
                                P      
     3.  Restriction data               
  <RET>  - enter chosen section P      
     3.1   Info screen                  
                                P      
     3.2   Enzymes screen               
  <F2>   - save current settingsP      
     3.3   New standards                
           and exit             P      
     3.4   Agarose gel                  
                                P      
     4.  Scan DNA sequence              
  <ESC>  - exit without saving  P      
     4.1   Active site selection        
                                P      
     4.2   Site selection info          
                                P      
     5.  Edit                           
                                P      
     5.1   Info screen                  
                                P      
     5.2   Cloning                      
                                P      
     6.  Batch screen                   
                                P      
     Default settings                   
                                P      
     Plotter drivers                    
                                
      
    P         SETUP  MENU  for the DRAWMAP program                                   P                                                                                P      
                                P      
     COLOR CUSTOMIZATION                
                                P      
                                P      
                                O      
                               
DMCUSTOM.SCR
Cannot find file DMCUSTOM.SCR
Cannot customize the program.
DRAWMAP.INIU
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